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The gels’ shelf life depends on the gel buffer’s polyacrylamide concentration and pH value. I.e. the lower the concentration, the longer the shelf life. Depending on gel type, a gel can have a shelf life of between six weeks to twelve months. However, we can assure you that all of the gels we supply always have a minimum shelf life of at least five weeks.

Wells’ holding capacities depends on the thickness of the gel and the width of the well, which decreases with increasing well quantity. The following table provides an overview of the wells’ holding capacity:

Layer thickness	10 strips	12 strips	15 strips
1.0 mm	25 µl	20 µl	15 µl
1.5 mm	37 µl	30 µl	22 µl

No, our sample buffers do not contain any reducing agents. You can therefore choose yourself whether to use, e.g. 2-mercaptothanol, DTT or DTE as a reducing agent. Reducing agents must be added immediately before a sample is denatured. Only use freshly reduced samples for electrophoresis. Please do not hesitate to contact us if you would like more information on this subject.

No, our transfer buffers do not contain any SDS or alcohol. SDS is necessary for the proteins’ mobility. After electrophoresis, the gel generally contains sufficient SDS to enable the proteins to be transferred to the membrane. Alcohol is often added to transfer buffers in order to remove SDS from the proteins and to increase the interactions between the nitrocellulose membrane and protein. However, this reduces the proteins’ mobility, reduces the gel matrix’s pore size and consequently makes the transfer harder. In some cases, it might nonetheless still be necessary to add SDS or alcohol to a transfer buffer, and this needs to be established experimentally. Please do not hesitate to contact us if you would like more information on this subject.